Krämer Lab
Crisci, A., Raleff, F., Bagdiul, I., Raabe, M., Urlaub, H., Rain, J.-C., and Krämer, A. (2015). Mammalian splicing factor SF1 interacts with SURP domains of U2 snRNP-associated proteins. Nucleic Acids Res. 43, 10456-10473. pubmed
Zhang, Y., Madl, T., Bagdiul, I., Kern, T., Kang, H.S., Zou, P., Mausbacher, N., Sieber, S.A., Krämer, A., and Sattler, M. (2013). Structure, phosphorylation and U2AF65 binding of the N-terminal domain of splicing factor 1 during 3'-splice site recognition. Nucleic Acids Res 41, 1343-1354. pubmed
Corioni, M., Antih, N., Tanackovic, G., Zavolan, M., and Krämer, A. (2011). Analysis of in situ pre-mRNA targets of human splicing factor SF1 reveals a function in alternative splicing. Nucleic Acids Res 39, 1868-1879. pubmed
Huang, C.J., Ferfoglia, F., Raleff, F., and Krämer, A. (2011). Interaction domains and nuclear targeting signals in subunits of the U2 small nuclear ribonucleoprotein particle-associated splicing factor SF3a. J Biol Chem 286, 13106-13114. pubmed
Tanackovic, G., and Krämer, A. (2005). Human splicing factor SF3a, but not SF1, is essential for pre-mRNA splicing in vivo. Mol Biol Cell 16, 1366-1377. pubmed
Krämer, A., Ferfoglia, F., Huang, C.J., Mulhaupt, F., Nesic, D., and Tanackovic, G. (2005). Structure-function analysis of the U2 snRNP-associated splicing factor SF3a. Biochem Soc Trans 33, 439-442. pubmed
Nesic, D., Tanackovic, G., and Krämer, A. (2004). A role for Cajal bodies in the final steps of U2 snRNP biogenesis. J Cell Sci 117, 4423-4433. pubmed
Kralovicova, J., Houngninou-Molango, S., Krämer, A., and Vorechovsky, I. (2004). Branch site haplotypes that control alternative splicing. Hum Mol Genet 13, 3189-3202. pubmed
Selenko, P., Gregorovic, G., Sprangers, R., Stier, G., Rhani, Z., Krämer, A., and Sattler, M. (2003). Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP. Mol Cell 11, 965-976. pubmed
Moreau, G., and Krämer, A. (2002). Splicing of nuclear pre-messenger RNAs (Pre-mRNAs). In Wiley Encyclopedia of Molecular Medicine, T. Creighton, ed. (New York: John Wiley & Sons, Inc.), pp. 2979-2982.
Liu, Z., Luyten, I., Bottomley, M., Messias, A., Houngninou-Molango, S., Sprangers, R., Zanier, K., Krämer, A., and Sattler, M. (2001). Structural basis for recognition of the intron branch site by splicing factor 1. Science 294, 1098-1102. pubmed
Nesic, D., and Krämer, A. (2001). Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation. Mol Cell Biol 21, 6406-6417. pubmed
Krämer, A., Grüter, P., Gröning, K., and Kastner, B. (1999). Combined biochemical and electron microscopic analyses reveal the architecture of the mammalian U2 snRNP. J Cell Biol 145, 1355-1368. pubmed
Mazroui, R., Puoti, A., and Krämer, A. (1999). Splicing factor SF1 from Drosophila and Caenorhabditis: presence of an N-terminal RS domain and requirement for viability. RNA 5, 1615-1631. pubmed
Wang, X., Bruderer, S., Rafi, Z., Xue, J., Milburn, P., Krämer, A., and Robinson, P. (1999). Phosphorylation of splicing factor SF1 on Ser-20 by cGMP-dependent protein kinase regulates spliceosome assembly. EMBO J 18, 4549-4559. pubmed
Krämer, A., Quentin, M., and Mulhauser, F. (1998). Diverse modes of alternative splicing of human splicing factor SF1 deduced from the exon-intron structure of the gene. Gene 211, 29-37. pubmed
Schmidt-Zachmann, M.S., Knecht, S., and Krämer, A. (1998). Molecular characterization of a novel, widespread nuclear protein that colocalizes with spliceosome components. Mol Biol Cell 9, 143-160. pubmed
Rain, J.-C., Rafi, Z., Rhani, Z., Legrain, P., and Krämer, A. (1998). Conservation of functional domains involved in RNA binding and protein-protein interactions in human and Saccharomyces cerevisiae pre-mRNA splicing factor SF1. RNA 4, 551-565. pubmed
Kennedy, C.F., Krämer, A., and Berget, S.M. (1998). A role for SRp54 during intron bridging of small introns with pyrimidine tracts upstream of the branch point. Mol Cell Biol 18, 5425-5434. pubmed
Schenkel, J., Jung, F., Guialis, A., and Krämer, A. (1998). In vitro splicing of pre-mRNAs in HeLa extracts. In Springer Lab Manual: RNP Particles, Splicing and Autoimmune Diseases, J. Schenkel, ed. (Heidelberg: Springer Verlag), pp. 184-210.
Krämer, A. (1996). The structure and function of proteins involved in nuclear pre-mRNA splicing. Annu Rev Biochem 65, 367-409. pubmed
Arning, S., Grüter, P., Bilbe, G., and Krämer, A. (1996). Mammalian splicing factor SF1 is encoded by variant cDNAs and binds to RNA. RNA 2, 794-810. pubmed
Rain, J.C., Tartakoff, A.M., Krämer, A., and Legrain, P. (1996). Essential domains of the PRP21 splicing factor are implicated in the binding to PRP9 and PRP11 proteins and are conserved through evolution. RNA 2, 535-550. pubmed
Krämer, A. (1995). The biochemistry of pre-mRNA splicing. In Pre-mRNA processing, A.I. Lamond, ed. (Austin: R.G. Landes Company), pp. 35-64.
Krämer, A., Mulhauser, F., Wersig, C., Gröning, K., and Bilbe, G. (1995). Mammalian splicing factor SF3a120 represents a new member of the SURP family of proteins and is homologous to the essential splicing factor PRP21p of S. cerevisiae. RNA 1, 260-272. pubmed
Krämer, A., Legrain, P., Mulhauser, F., Gröning, K., Brosi, R., and Bilbe, G. (1994). Splicing factor SF3a60 is the mammalian homologue of PRP9 of S. cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo. Nucleic Acids Res 22, 5223-5228. pubmed
Brosi, R., Hauri, H.P., and Krämer, A. (1993). Separation of splicing factor SF3 into two components and purification of SF3a activity. J Biol Chem 268, 17640-17646. pubmed
Brosi, R., Gröning, K., Behrens, S.-E., Lührmann, R., and Krämer, A. (1993). Interaction of mammalian splicing factor SF3a with U2 snRNP and relation of its 60-kD subunit to yeast PRP9. Science 262, 102-105. pubmed
Krämer, A. (1993). Mammalian protein factors involved in nuclear pre-messenger RNA splicing. Mol Biol Rep 18, 93-98.
Krämer, A. (1992). Purification of splicing factor SF1, a heat-stable protein that functions in the assembly of a pre-splicing complex. Mol Cell Biol 12, 4545-4552. pubmed
Utans, U., Behrens, S.-E., Lührmann, R., Kole, R., and Krämer, A. (1992). A splicing factor that is inactivated during in vivo heat shock is functionally equivalent to the [U4/U6.U5] triple snRNP-specific proteins. Genes Dev. 6, 631-641. pubmed
Nemeroff, M.E., Utans, U., Krämer, A., and Krug, R.M. (1992). Identification of cis-acting intron and exon regions in Influenza virus NS1 mRNA that inhibit splicing and cause the formation of aberrantly sedimenting presplicing complexes. Mol Cell Biol 12, 962-970. pubmed
Krämer, A., and Utans, U. (1991). Three protein factors (SF1, SF3 and U2AF) function in pre-splicing complex formation in addition to snRNPs. EMBO J. 10, 1503-1509. pubmed
Utans, U., and Krämer, A. (1990). Splicing factor SF4 is dispensable for the assembly of a functional splicing complex and participates in the subsequent steps of the splicing reaction. EMBO J 9, 4119-4126. pubmed
Krämer, A. (1990). Purification of small nuclear ribonucleoprotein particles active in RNA processing. In Meth. Enzymol., J.E. Dahlberg, and J.N. Abelson, eds. (New York: Academic Press), pp. 215-232.
Krämer, A. (1990). Site-specific degradation of RNA of small nuclear ribonucleoprotein particles with complementary oligodeoxynucleotides and RNase H. In Methods Enzymol., J.E. Dahlberg, and J.N. Abelson, eds. (New York: Academic Press), pp. 284-292.
Krämer, A., and Keller, W. (1990). Preparation and fractionation of mammalian extracts active in pre-mRNA splicing. In Meth Enzymol, J.E. Dahlberg, and J.N. Abelson, eds. (New York: Academic Press), pp. 3-19.
Krämer, A. (1988). Pre-splicing complex formation requires two proteins and U2 snRNP. Genes Dev 2, 1155-1167. pubmed
Steitz, J.A., Black, D.L., Gerke, V., Parker, K.A., Krämer, A., Frendewey, D., and Keller, W. (1988). Functions of the abundant U-snRNPs. In Structure and Function of Major and Minor Small Nuclear Ribonucleoprotein Particles, M.L. Birnstiel, ed. (Berlin: Springer-Verlag), pp. 115-154.
Krämer, A., Frick, M., and Keller, W. (1987). Separation of multiple components of HeLa cell nuclear extracts required for pre-messenger RNA splicing. J Biol Chem 262, 17630-17640. pubmed
Krämer, A. (1987). Fractionation of HeLa cell nuclear extracts reveals minor small nuclear ribonucleoprotein particles. Proc Natl Acad Sci U S A 84, 8408-8412. pubmed
Krämer, A. (1987). Analysis of RNase-A-resistant regions of adenovirus 2 major late precursor-mRNA in splicing extracts reveals an ordered interaction of nuclear components with the substrate RNA. J Mol Biol 196, 559-573. pubmed
Frendewey, D., Krämer, A., and Keller, W. (1987). Different small nuclear ribonucleoprotein particles are involved in different steps of splicing complex formation. Cold Spring Harb Symp Quant Biol 52, 287-298.
Gick, O., Krämer, A., Vasserot, A., and Birnstiel, M.L. (1987). Heat-labile regulatory factor is required for 3' processing of histone precursor mRNAs. Proc Natl Acad Sci U S A 84, 8937-8940. pubmed
Gick, O., Krämer, A., Keller, W., and Birnstiel, M.L. (1986). Generation of histone mRNA 3' ends by endonucleolytic cleavage of the pre-mRNA in a snRNP-dependent in vitro reaction. EMBO J 5, 1319-1326. pubmed
Krämer, A., and Keller, W. (1985). Purification of a protein required for the splicing of pre-mRNA and its separation from the lariat debranching enzyme. EMBO J. 4, 3571-3581. pubmed
Krämer, A. (1985). 5S ribosomal gene transcription during Xenopus oogenesis. In Developmental Biology: A comprehensive synthesis, L.W. Browder, ed. (New York: Plenum Publishing Corporation), pp. 431-451.
Krämer, A., Keller, W., Appel, B., and Lührmann, R. (1984). The 5' terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors. Cell 38, 299-307. pubmed
Krämer, A., and Roeder, R.G. (1983). The use of monoclonal antibodies for the characterization of a 5 S gene-specific transcription factor (IIIA) from Xenopus laevis. J Biol Chem 258, 11915-11923. pubmed
Krämer, A., and Bautz, E.K. (1981). Immunological relatedness of subunits of RNA polymerase II from insects and mammals. Eur. J. Biochem. 117, 449-455. pubmed
Krämer, A., Haars, R., Kabisch, R., Will, H., Bautz, F.A., and Bautz, E.K. (1980). Monoclonal antibody directed against RNA polymerase II of Drosophila melanogaster. Mol Gen Genet 180, 193-199. pubmed
Greenleaf, A.G., Krämer, A., and Bautz, E.K.F. (1976). DNA dependent RNA polymerases from Drosophila melanogaster larvae. In RNA polymerase, R. Losick, and M. Chamberlin, eds. (New York: Cold Spring Harbor Laboratory Press), pp. 793-801.